If a spore test comes back positive, how long must you wait until the autoclave can be used again?

A positive spore test indicates that the autoclave is not functioning properly and may not be effectively sterilizing instruments. Therefore, before the autoclave can be used again for sterilization, it is critical to receive a negative spore test result from three consecutive testing cycles. This protocol ensures the autoclave's ability to consistently kill all microbial life, including spores, which are resistant forms of bacteria.

Waiting for three consecutive negative spore tests confirms that any issues with the autoclave have been resolved, providing a higher level of assurance for safety and efficacy in the sterilization process. This rigorous testing is part of infection control procedures to maintain a safe environment in any practice that relies on sterilization.

Other options may suggest a shorter timeframe or different conditions, but they do not align with best practices for ensuring sterilization safety after a positive spore test.